Cycling temperature treatments and digestion in prairie lizards (Sceloporus consobrinus)

In nature, many organisms experience a daily range of body temperatures. Thermal performance at stable temperatures is often extrapolated to predict function in cyclical environments. However, temperature order and cyclicity may influence physiological processes. The current study compared energy intake, digestive passage time, and energy budgets across stable temperature (30˚C, 33˚C, 36˚C) and two temperature cycles in lizards (Sceloporus conosbrinus), determining 1) if stable treatments adequately project performance in a cycling environment and 2) if temperature order influences performance. Cycles rotated through 30˚C, 33˚C, 36˚C daily, with equal durations of time at each temperature but differing temperature order, with warm days and cool nights in cycle 1 and cool days and warm nights in cycle 2. For analyses, stable treatments were compiled into a single dataset and compared to cycles. If temperature is the primary factor regulating performance, then performance averages from st..., Field Collection            Male and female adult S. consobrinus were collected in northwest Arkansas from 2020 – 2022, ranging in size from 48 – 70mm snout-vent length (n = 39). All necessary permits were acquired for the research conducted (IACUC #19080, Arkansas Game and Fish Commission Permit #050120211). Temperature Treatments Lizards were randomly assigned to one of three temperature treatments, stable 33°C (n = 12), cycle 1 (n = 12), or cycle 2 (n = 15), with males and females as evenly divided as possible. The first cycling temperature treatment (referred to as cycle 1) consisted of a nighttime temperature of 30°C and a daytime temperature of 36°C, with a sunrise and sunset temperature of 33°C during the transition between the low and high temperatures. To compare performance under different orders of temperature cycles the second cycling temperature treatment (referred to as cycle 2) consisted of a nighttime temperature of 36°C and a daytime temperature of 30°C, with a sunris..., , # Cycling Temperature Treatments and Digestion in Prairie Lizards (Sceloporus consobrinus) The dataset contains all information used in the analysis. Physiological data were collected at 33C stable temperature, and under two cycling temperature treatments. We looked at food consumption rate using voluntary consumption with crickets, in first grams which was converted to kilojoules. We also quantified digestive passage time in hours, the time from consuming food to excreting it. We quantified energy excreted in grams which was converted to kilojoules, for both urates and feces. Lastly, we calculated metabolizable energy intake and assimilated energy considering food consumed (kJ) and excreted (kJ). ## Description of the data and file structure The columns are titled according to the data contained: * Lizard - This column is a unique ID indicating which lizard was used. These are specific to our lab, but indicate each lizard was unique. * Temperature - Temperature refers to the ...自然界中，诸多生物的体温会呈现每日周期性波动。以往研究常通过恒温环境下的热性能表现外推，以预测周期性温度环境中的生物功能。然而，温度时序与周期性或许会对生理过程产生影响。本研究针对草原蜥蜴（Sceloporus consobrinus），比较了恒温环境（30℃、33℃、36℃）与两种温度周期环境下的能量摄入、消化通过时间（digestive passage time）及能量收支（energy budgets），旨在明确两个问题：1）恒温处理是否能够准确预测周期性温度环境中的生物性能；2）温度时序是否会影响生物性能。两种温度周期均每日在30℃、33℃、36℃之间切换，各温度下的持续时长一致，但温度时序不同：周期1为昼暖夜凉，周期2为昼凉夜暖。分析时，将所有恒温处理的数据整合为单一数据集，并与周期处理数据进行比较。若温度是调控生物性能的核心因素，则恒温下的性能平均值应能... 野外采样 于2020年至2022年间，在阿肯色州西北部采集成年草原蜥蜴（S. consobrinus）雌雄个体，其吻肛长（snout-vent length）介于48~70mm之间，共39只。本研究已获取所有必要的科研许可（实验动物护理与使用委员会许可编号IACUC #19080，阿肯色州鱼类与野生动物委员会许可编号050120211）。 温度处理方案 将受试蜥蜴随机分配至三种温度处理组：恒温33℃组（n=12）、周期1组（n=12）与周期2组（n=15），各组雌雄个体分配比例尽可能保持均衡。第一种循环温度处理（下称周期1）的夜间温度为30℃，日间温度为36℃，高低温转换阶段的日出、日落过渡温度为33℃。为对比不同温度时序循环下的生物性能，第二种循环温度处理（下称周期2）的夜间温度为36℃，日间温度为30℃，日出过渡温度... # 草原蜥蜴（Sceloporus consobrinus）的循环温度处理与消化 本数据集包含了本研究分析所用的全部信息。生理数据采集自恒温33℃环境与两种循环温度处理环境。本研究采用蟋蟀饲喂法测定蜥蜴的自愿摄食率，初始摄食量以克为单位，后转换为千焦。同时量化了消化通过时间（digestive passage time），即从摄食至排泄的时长，单位为小时。针对尿酸盐与粪便分别量化了排泄能量，初始单位为克，后转换为千焦。最终，基于摄食总能量（千焦）与排泄总能量（千焦），计算得到可代谢能量摄入（metabolizable energy intake）与同化能量（assimilated energy）。 数据与文件结构说明 各数据列的标题与所含内容对应如下： * Lizard：该列存储唯一标识编号，用于区分每只受试蜥蜴。本编号体系为本实验室专属，每条记录均对应唯一的受试个体。 * Temperature：温度列指的是...