Transcriptomic Profiling of Ferroptosis in Enteric Neurons: Bulk RNA-Seq of IM-FEN Cells Treated with Palmitate

Palmitic acid (PA), a major component of Western dietary fats, is known to induce ferroptotic cell death in enteric neurons. In this study, we performed bulk RNA-seq on Immortalized Mouse-Fetal Enteric Neurons (IM-FEN) treated with PA (0.5 mM, 24 h) or vehicle control to characterize transcriptional changes associated with ferroptosis. Sequencing was performed using the Illumina NovaSeq 6000 platform. The dataset provides insight into lipid-induced neuronal stress and ferroptosis-relevant signaling networks. Overall design: IM-FEN cells were treated with palmitic acid (0.5 mM) or vehicle control (10% BSA) for 24 hours. Each condition included six biological replicates (n = 6). Total RNA was extracted and subjected to paired-end sequencing on the Illumina NovaSeq 6000 platform. The study includes 12 raw FASTQ files (paired-end; R1 and R2) and a normalized gene expression matrix. This dataset enables transcriptomic comparison of ferroptosis-associated gene programs induced by lipid overload in enteric neurons.