Antiviral innate immunity of hepatitis C virus-infected stem cell-derived hepatocytes

Purpose: RNAseq analysis of hPSC undergoing in vitro hepatic differentiation, to validate proper differentiation at different times of differentiation (D0 to D21) Overall design: Differentiated hepatocytes were then subjected to HCV infection (HCV compared to non infected NINF control), with or without various antiviral treatment (TEL) or JAK/STAT pathway inhibitor treatment (JSI), followed by analysis of global gene expression change. Particular emphasize was done on Innate Immune Genes and Interferon Regulated Genes (IRG) For each sample, individual run. hPSC undergoing in vitro hepatic differentiation. Differentiated hepatocytes subjected to HCV infection (HCV compared to non infected NINF control), with or without various antiviral treatment (TEL) or JAK/STAT pathway inhibitor treatment (JSI).