Elevated sleep quota in a stress-resilient Drosophila species

Sleep is broadly conserved across the animal kingdom but can vary widely between species. It is currently unclear which selective pressures and regulatory mechanisms influence differences in sleep between species. The fruit fly Drosophilamelanogaster has become a successful model system for examining sleep regulation and function, but little is known about the sleep patterns in many related fly species.  Here, we find that fly species with adaptations to extreme desert environments, including D. mojavensis, exhibit strong increases in baseline sleep compared to D. melanogaster. Long-sleeping D. mojavensis show intact homeostasis, indicating that desert flies carry an elevated drive for sleep. In addition, D. mojavensis exhibit altered abundance or distribution of several sleep/wake related neuromodulators and neuropeptides that are consistent with their reduced locomotor activity and increased sleep. Finally, we find that in a nutrient-deprived environment, the sleep patterns of individ..., Behavior
4-8 day old female flies were housed individually in borosilicate glass tubes (65mm length, 5mm diameter) containing fly food coated with paraffin wax at one end and a foam plug in the other. Locomotor activity was recorded using DAM5M or DAM5H multibeam Drosophila Activity Monitors from Trikinetics Inc. (Waltham MA, USA) and sleep was analyzed in Matlab (MathWorks Inc) with the SCAMP script package90. Locomotor activity was measured as the number of movements between beams per one-minute bins. Periods of sleep were defined by at least 5 minutes with no change in position within the multibeam activity monitors. Sleep time courses display 30-min time bins.
Sleep Deprivation and Arousability
Sleep deprivations were performed mechanically by mounting DAM5M activity monitors onto platform vortexers (VWR 58816-115). Individual tubes were plugged with food at one end and 3D-printed PLA plastic caps at the other. Monitors were vortexed at an intensity of 2.5g for 3-second pulses ever..., , # Elevated sleep quota in a stress-resilient Drosophila species

[https://doi.org/10.5061/dryad.w9ghx3fxw](https://doi.org/10.5061/dryad.w9ghx3fxw)

Dataset contains underlying data for main and supplemental figures from manuscript.

Included files:

1. Source data for Figure 1.xls
1. Baseline sleep time course data (Figure 1A), total day and night sleep (Figure 1B; values in minutes of sleep), P(wake) (Figure 1C), P(doze) (Figure 1D), and counts/waking minute (Figure 1E) for females from D. melanogaster and D. mojavensis stocks. Columns represent data from individual flies with stock label in Row 1. Rows represent 30-min time bins for Figure 1A, or day and night for Figures 1B-E.
2. Length of individual sleep bouts during the day (Figure 1F) and night (1G) from D. melanogaster and D. mojavensis stocks.
3. Daily sleep amount for D. melanogaster and D. mojavensis stocks using different time durations for minimum sleep bout length (Figure 1H; values in minutes of sleep).
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