Single cell RNA-sequencing on E16.5 and E17.5 mouse lungs

To identify clear mesenchymal cell clusters, sequencing libraries of embryonic lung cells were prepared using the 10x Genomics Chromium system. After quality control samples were integrated. The cells were visualized in two dimensions according to their gene expression profiles using Uniform Manifold Approximation and Projection (UMAP). We FACS sorted alphaSMA-GFP; Tbx4-Cre; Rosa26-tdTomato (Tbx4-lineage+,alphaSMA+) fibroblasts from one E16.5 mouse lung and live cells from three E17.5 murine lungs. Single cell RNA-seq was performed on purified single cells.