Gene expression profiles of active and restricted R/G-HIV+ primary human fetal astrocytes

The gene expression patterns of astrocyte harboring active vs restricted LTRs were similar, and that the active population demonstrated more widespread and robust changes. Infection of astrocytes with VSVG pseudotyped Red/Green-HIV-1 wildtype and integrase (D116A) mutant. Astrocytes were sorted into latent (R+/G-), active (R+/G+), D116A (R-/G-) and untreated two weeks post-infection. RNA was isolated and sequenced in three separate donors.