Comparison of hepatocarcinogen-induced gene expression profiles in conventional primary rat hepatocytes with in vivo rat liver

At present, substantial efforts are focused on the development of in vitro assays coupled with ”omics” technologies for the identification of carcinogenic substances as an alternative to the classical 2-year rodent carcinogenicity bioassay. A prerequisite for the eventual regulatory acceptance of such assays, however, is the in vivo relevance of the observed in vitro findings.当前，大量研究工作已聚焦于开发结合组学（omics）技术的体外实验（in vitro assay），用于识别致癌物质，以此作为经典两年期啮齿类动物致癌性生物试验的替代方案。然而，此类检测方法最终获得监管机构认可的先决条件，是其所观测到的体外实验结果具备体内（in vivo）相关性。