Structural snapshots of the mechanism of ATP-dependent DNA damage recognition by UvrA

This repository includes the following source datasets: 1. 2-AP_fluorescence_3.zip This contains the following files:1. The full spectra (340nm-440 nm) for all the samples provided in text (.txt) files.2. The values recorded at 370 nm recorded in the excel sheet “Values_v2.xlsx”. 3. Details of the files in the folder. 2. UvrB loading experiments_v04.zip This contains the following files: The gel images (.tif) along with the raw gels images (.raw16.tif) for SDS-PAGE (Coomassie stained) and TBE-Urea PAGE (Ethidium bromide stained), for three independent experiments, for the flu-dT-DNA and unmodified-DNA, for the following combinations of proteins(i) Ct-UvrA (WT)- Ct-UvrB (K44A)(ii) Ct -UvrA (Y62A)- Ct -UvrB (K44A) The data used for quantification [excel files “Densitometry-10x elution triplicates_WT_Y62A” and “exported_data” and the corresponding TBE-Urea PAGE images (.tif and .raw16.tif)] Details of the files in the folder. 3. Incision_activity.zip This contains TBE-Urea PAGE images (.gel and .tif) with incision activity reactions for Uvr proteins for three independent experiments and a word file with the details. 4. UvrA-UvrB-UvrC_proteins.zip This contains the following files: The gel images (.tif) along with the raw gels images (.raw16.tif) for SDS-PAGE (Coomassie stained) for(i) Ct-UvrA wildtype and variant proteins (with His-tag and without tag)(ii) Ct-UvrB wildtype and variant proteins (with His-tag and without tag)(iii) Ct-UvrC wildtype protein with His-tag and Tm-UvrC without tag Details of the files in the folder.