GmRAV1 regulates regeneration of root and adventitious buds by cytokinin in Arabidopsis and soybean

Purpose: The goals of this study are to identify what gene GmRAV1 was related to. RNA-Sequencing experiments were designed between GmRAV1-ox soybeans and DN50 soybeans.Methods: Leaves mRNA of 30-day-old DN50 and GmRAV1-ox soybeans were used by RNA sequencing using Illumina HiSeqTM2000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with TopHat followed by Cufflinks. Genome_build: Glycine max Wm82.a2.v1Results: RNA-seq data confirmed that there are 1467 genes with significant differences. Some genes expression of cytokinin and auxin signaling pathways is dramatically changed, such as GmARR9, GmAUX22, GmPIN1, GmCycD3 and GmPDV2.Conclusions: GmRAV1 are related to cytokinin and auxin signaling pathways.

研究目的：本研究旨在明确基因GmRAV1所参与的生物学通路与调控关联。本实验以GmRAV1过表达大豆与DN50大豆为材料开展RNA测序（RNA-Sequencing）实验。
方法：选取生长30天的DN50大豆与GmRAV1过表达大豆的叶片mRNA，采用Illumina HiSeq™2000平台开展RNA测序。对通过质量过滤的测序读段，依次采用TopHat与Cufflinks工具在转录本异构体水平进行数据分析。
参考基因组版本：大豆（Glycine max）Wm82.a2.v1
结果：RNA测序数据证实共鉴定得到1467个差异表达基因。细胞分裂素与生长素信号通路中的部分基因的表达水平发生显著改变，包括GmARR9、GmAUX22、GmPIN1、GmCycD3及GmPDV2。
结论：GmRAV1与细胞分裂素及生长素信号通路密切相关。