Angiogenic, apoptotic and autophagic profiling of chronic myeloid leukaemia patients’ platelets ex vivo before and after treatment with Imatinib

The data presented here was to
determine the angiogenic, apoptotic, and autophagic profiles of chronic myeloid
leukaemia patients ex vivo on
platelets before and after treatment with Imatinib. Platelet counts, viability and activation were determined by human cluster of
differentiation (CD) 41 and -62 markers. The possible occurrence of apoptosis
and autophagy was measured by means of flow cytometry and western blot analysis
via Annexin V-fluorescein isothiocyanate (FITC), caspase 3, autophagy related gene
5 (Atg5) and light chain I and II (LC3) markers. Angiogenesis expression was
determined through vascular endothelial growth factor (VEGF) and transforming
growth factor β (TGFβ) markers.