Sulfur assimilation improves SAM-dependent methylation

We employed omic data and conducted comparative analysis in Saccharomyces cerevisiae strains with different S-adenosine-L-methionine (SAM) level. This strategy can help us locate the limiting factor for SAM availability. We found sulfur assimilation emerged as the most significantly enriched pathway and further confirmed that sulfur assimilation was the dominant limiting step of SAM biosynthesis in the whole cell system.本研究采用组学数据，对不同S-腺苷-L-甲硫氨酸（S-adenosyl-L-methionine，SAM）水平的酿酒酵母（Saccharomyces cerevisiae）菌株开展比较分析。该策略可辅助我们定位影响SAM供给的限制性因素。研究发现硫同化途径为最显著富集的通路，并进一步证实，在全细胞系统中，硫同化是SAM生物合成的核心限制性步骤。