Does mechanical damage on soybean induces the production of flavonoids?

Abstract The response of plants to grazing includes the production of chemical defense compounds such as proteases inhibitors and secondary metabolites as flavonoids, which makes them less palatable to feeding and negatively affecting the physiology of insects. The aim of this study was to evaluate the phytochemical response of soybean cultivars (Glycine max (L.) Merrill) resistant (IAC-17, IAC-24) and susceptible (IAC-P1) to insects after mechanical damage. These cultivars were mechanically injured, and after 24 hours samples of these plants were analyzed by HPLC to identify and quantify flavonoids. The flavonoids daidzein, quercetin, and rutin were quantified, with the highest concentration of daidzin in soybean cultivars after mechanical damage. Rutin was biosynthesized by IAC-24. The cultivars IAC-PL1, IAC-17, and IAC-24 did not show a flavonoid response to mechanical damage. The soybean cultivars are not dependent on mechanical damage to produce flavonoids.摘要 植物对放牧的响应包括合成蛋白酶抑制剂、类黄酮（flavonoids）等次生代谢物类化学防御物质，此类物质可降低植株的适口性，并对取食昆虫的生理机能产生负面影响。本研究旨在评估机械损伤后，抗虫品种（IAC-17、IAC-24）与感虫品种（IAC-P1）大豆（Glycine max (L.) Merrill）的植物化学响应。研究人员对上述品种施加机械损伤处理，并在24小时后采集植株样本，通过高效液相色谱法（High Performance Liquid Chromatography, HPLC）对样本进行分析，以鉴定并定量类黄酮成分。本研究定量检测了大豆苷元（daidzein）、槲皮素（quercetin）与芦丁（rutin）三类类黄酮，结果显示机械损伤后的大豆品种中大豆苷（daidzin）浓度最高。芦丁仅由IAC-24品种生物合成。IAC-PL1、IAC-17与IAC-24三个品种均未在机械损伤后表现出类黄酮响应。大豆品种合成类黄酮并不依赖机械损伤刺激。