Illumina MiSeq amplicons of the V4 hyper variable region of 16S rRNA gene from IODP Expedition 336 on R/V JOIDES Resolution in the Mid-Atlantic Ridge in 2011 (North Pond basalts project)
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<p>This dataset contains Illumina MiSeq amplicons of the V4 hyper variable region of 16S rRNA gene. Raw data have been deposited in the NCBI Sequence Read Archive under accession number <a href=\"http://trace.ncbi.nlm.nih.gov/Traces/sra/?study=SRP068428\" target=\"_blank\">SRP068428</a>.</p>
<p><strong>Additional data files:</strong><br />
<strong>(1)</strong> FASTA-formatted quality-controlled 16s rRNA gene sequences, organized by name of the operational taxonomic unit (OTU): <a href=\"http://dmoserv3.whoi.edu/data_docs/C-DEBI/orcutt/X336_DataReport_Supplemental_filteredOTUs.fasta\" target=\"_blank\">X336_DataReport_Supplemental_filteredOTUs.fasta</a><br />
<strong>(2)</strong> Excel file containing a record of the abundance of each OTU in each sample, organized by the name of the IODP Expedition 336 sample, along with the taxonomy of the OTU (based on classification as described in methods) and the closest environmental relative as identified using NCBI's BLAST algorithm (as described in methods): <a href=\"http://dmoserv3.whoi.edu/data_docs/C-DEBI/orcutt/X336_DataReport_Supplemental_SequenceAbundance.xlsx\" target=\"_blank\">X336_DataReport_Supplemental_SequenceAbundance.xlsx</a></p>
<p>本数据集包含16S rRNA基因(16S ribosomal RNA gene)V4高变区的Illumina MiSeq扩增子。原始数据已提交至美国国家生物技术信息中心(National Center for Biotechnology Information, NCBI)的序列读取档案(Sequence Read Archive, SRA),登录编号为<a href="http://trace.ncbi.nlm.nih.gov/Traces/sra/?study=SRP068428" target="_blank">SRP068428</a>。</p>
<p><strong>补充数据文件:</strong><br />
<strong>(1)</strong> FASTA格式的质控后16S rRNA基因序列,按操作分类单元(operational taxonomic unit, OTU)名称进行组织:<a href="http://dmoserv3.whoi.edu/data_docs/C-DEBI/orcutt/X336_DataReport_Supplemental_filteredOTUs.fasta" target="_blank">X336_DataReport_Supplemental_filteredOTUs.fasta</a><br />
<strong>(2)</strong> 包含各样本中每个OTU丰度记录的Excel文件,以国际大洋发现计划(International Ocean Discovery Program, IODP)336航次的样本名称进行整理,同时包含OTU的分类学信息(基于文中所述方法的分类结果)以及通过NCBI的基本局部比对搜索工具(Basic Local Alignment Search Tool, BLAST)算法鉴定得到的最近环境亲缘种(方法详见文中描述):<a href="http://dmoserv3.whoi.edu/data_docs/C-DEBI/orcutt/X336_DataReport_Supplemental_SequenceAbundance.xlsx" target="_blank">X336_DataReport_Supplemental_SequenceAbundance.xlsx</a></p>
创建时间:
2022-04-15



