Lactate dehydrogenase release (LDH, %), caspase-3 activity (CAS, nmol per mg of protein per hr) and chromatin cleavage (CHR, %) in cells treated with ouabain, K+-free and Ca2+-free medium during 4 hr.
收藏Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Lactate_dehydrogenase_release_LDH_caspase_3_activity_CAS_nmol_per_mg_of_protein_per_hr_and_chromatin_cleavage_CHR_in_cells_treated_with_ouabain_K_free_and_Ca_2_free_medium_during_4_hr_/303957
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To measure LDH release and chromatin cleavage, the total content of LDH and [3H]-labelled DNA were taken as 100%. Ouabain was added at final concentration of 3 µM (HeLa and HUVEC) or 3 mM (RVSMC). Ca2+-free medium contained 50 µM EGTA and 10 µM BAPTA-AM. Staurosporine, a potent trigger of apoptosis, was added as a positive control at concentration of 1 µM. Means ± S.E. from experiments performed with quadruplicate are given.*p
创建时间：
2015-12-02
