Mechanomics RT-DC Set 05 - iPSCs

Entry is a part of Mechanomics collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript:
Urbanska, M., Ge, Y. et al.,
De novo identification of universal cell mechanics gene signatures
BiorXiv: https://doi.org/10.1101/2021.04.26.441418; first published: 27th April 2021, last updated: 4th Nov 2024
eLife: https://doi.org/10.7554/eLife.87930; first published: 23rd March 2023, last updated: 27th Dec 2024

This dataset contains mechanical measurements of murine induced pluripotent stem cells (iPSCs) of two different classes:
• transgene-dependent, fuzzy-colony forming - F-class
• transgene-independant, compact-colony forming - C-class.

These two cell types were first characterised by Tonge et al (Nature, 2014). The cell lines used in this study were derived in a previously published study by Urbanska et al (Development, 2017) via reprogramming of neural progenitor cells using a dox-induced expression of the OSKM reprogramming factors.
Measurements were performed using real-time deformability cytometry (RT-DC).
_rtdc.zip - contains data acquired during the measurments in '*.rtdc' format
_tsv.zip - contains extended data in a '*.tsv' format
_glmm.zip - contains result summaries of statistical analysis comparing Young's moduli of the different cell types using generalised linear mixed effects models
Tonge, P. D. et al. Divergent reprogramming routes lead to alternative stem-cell states. Nature 516, 192–197 (2014) https://doi.org/10.1038/nature14047
Urbanska, M. et al. Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage. Development 144, 4313–4321 (2017) https://doi.org/10.1242/dev.155218