RNA-Seq on the effect of VitaminD on colonic and tumor organoids

Stem cell enriched cultures are generated from primary cultures of colorectal cancer patients. Organoids from normal and tumoral tissue are treated with 1,25(OH)2D3 and vehicle for 96 hours. RNA expression is compared among treatments. Paired samples of healthy and tumoral tissue are obtained from six colorectal cancer patients. After enzymatic and mechanic tissue disgregation 3D cultures were established and treated with 1,25(OH)2D3 and vehicle for 96 hours. Treated organoids from colonic and tumorlal tissue are RNA-sequenced. UPDATE: [02-07-2024] The Sample titles, tissue characteristics, and treatment characteristics were corrected. The raw data and processed data associated with each Sample GSMxxx accession number did not change.