Identification of p66Shc promoter interactional DNA fragments using Circularized Chromosome Conformation Capture coupled with next-generation sequencing (4C-seq)

p66Shc is encoded by the SHC1 gene, which contains two tandem promoters that generate three proteins (p46, p52 and p66). More recently, p66Shc was found to localize to focal adhesions that reports cell attachment and is required for anoikis through a RhoA-dependent mechanosensory test (Ma et al., J Cell Biol, 179, 23-31). Consistent with its role in anoikis, p66Shc is expressed in normal epithelial and endothelial cells but not in hematopoietic cells that survive in unanchored conditions. To uncover the epigenetic regulation mechanisms of p66Shc expression, here we performed 4C-seq assays with p66Shc promoter as a bait in HUVEC and U937 cells. Our results identify different p66Shc promoter interactional DNA fragments in different cell types. Overall design: 4C-seq analysis of p66Shc promoter with putative interactional DNA fragments in HUVEC and U937 cells.