RNA-Seq of wild-type (WT) and smn2 mutants for quantitative analysis of transcriptomes and mapping SMN2-dependent degraded regions

To compare expression of defense-related genes between WT and smn2 mutant, we performed the RNA-seq analysis using the total RNA prepared from Arabidopsis WT and smn2 seedlings. RNA-seq profiling showed 250 Differentially Expressed Genes (DEGs) detected in smn2 compared to WT. Genome-wide mapping of DEG regions was also performed to elucidate SMN2-dependent degraded regions in defense-related genes. Overall design: Fourteen-day old WT and smn2 seedlings were treated with 0.1 uM flg22 for 0hr or 4hr with two biological replicates.