A microarray analysis of human epidermal keratinocytes upon depletion of the long non-coding RNA LOC100130476

The lncRNA LOC100130476 (named as WAKMAR2) was found to be down-regulated in epidermal keratinocytes in human chronic non-healing wounds compared to normal acute wounds and the intact skin. However, its biological role in keratinocytes during wound repair has not been studied. To study the genes regulated by WAKMAR2, we transfected Antisense LNA GapmeR against WAKMAR2 into human primary epidermal keratinocytes to deplete it. We performed a transcriptome-wide gene expression level profiling of keratinocytes upon depletion of WAKMAR2 using Affymetrix arrays. Expression profiling of primary human epidermal keratinocytes transfected with 20nM WAKMAR2-specific GapmeR or 20nM GapmeR negative control for 24 hours (biological triplicates in each group) was performed using Affymetrix GeneTitan system.