Dynamic DNA methylation reveals novel cis-regulatory elements in murine hematopoiesis

Genome wide DNA methylation profiling of 9 different cell types of the murine hematopoietic system. The samples comprise FACS-sorted biological triplicates of hematopoietic stem and progenitor cells (LSK, CMP, GMP, MEP) and terminally differentiated immune cells (monocytes, neutrophils, CD4+ T-cells, CD8+ T-cells, B-cells). Additionally, bone marrow of CD45.1 (B6.SJL-Ptprca Pepcb/BoyCrl) and CD45.2 (C57BL/6J) mice as well as JAK2-V617F mutant mice was analyzed. The Illumina Infinium Mouse Methylation BeadChip was used to generate DNA methylation profiles across approximately 285,000 CpGs in these samples. DNA methylation profiles were generated from 9 different FACS-sorted murine hematopoietic cell types isolated from bone marrow of two congenic mouse strains (CD45.1, CD45.2) and of JAK2-V617F mutant mice. DNA methylation data was generated using the Illumina Infinium Mouse Methylation BeadChip array.