DUF4297 and HerA form abortosome to mediate bacterial immunity against phage infection. Dongmei Tang et al.

Figure1. 1D Cell viability assessed for strains expressing EcDUF4297‒EcHerA, EcDUF4297 alone, or EcHerA alone by serial dilutions. 1G The nuclease and ATPase activities of DUF4297‒HerA. 1H Hailibu degrades phage genome, bacterial genome, and PCR product. 1J The in vivo nuclease activity of Hailibu system.
Figure3. 3B Sequencing raw data of DNA degradation products by Hailibu abortosome.
Figure S1. S1A. Degradation of genome DNA of P1-infected E. coli cells in the presence of EcHailibu system. S1B.The nuclease activity of EcHailibu under various ATP concentrations. S1C. The nuclease activity of EcHailibu with various adenosine nucleotides. S1D. Thin-layer chromatography analysis of EcHailibu defense system ATPase against ATP.
Figure S3. S3A.The nuclease activity of AtHailibu. S3B. The nuclease and ATPase activities of AtHailibu. S3C. AtHailibu degrades phage genome, bacterial genome, and PCR product. S3D.The nuclease activity of AtHailibu under various ATP concentrations. S3E. The nuclease activity of AtHailibu with various adenosine nucleotides. S3G.Thin-layer chromatography analysis of the ATPase activity of AtHailibu.

图1. 对表达EcDUF4297-EcHerA、仅EcDUF4297或仅EcHerA的菌株进行1D细胞活力评估，通过连续稀释进行。图1G. DUF4297-HerA的核酸酶和ATP酶活性。图1H. Hailibu降解噬菌体基因组、细菌基因组及PCR产物。图1J. Hailibu系统的体内核酸酶活性。图3. 3B. Hailibu abortosome降解DNA降解产物的测序原始数据。图S1. S1A. 在EcHailibu系统的存在下，P1感染的大肠杆菌细胞的基因组DNA降解。S1B. EcHailibu在各种ATP浓度下的核酸酶活性。S1C. EcHailibu与各种腺苷核苷的核酸酶活性。S1D. EcHailibu防御系统ATP酶对ATP的薄层色谱分析。图S3. S3A. AtHailibu的核酸酶活性。S3B. AtHailibu的核酸酶和ATP酶活性。S3C. AtHailibu降解噬菌体基因组、细菌基因组及PCR产物。S3D. AtHailibu在不同ATP浓度下的核酸酶活性。S3E. AtHailibu与各种腺苷核苷的核酸酶活性。S3G. AtHailibu的ATP酶活性的薄层色谱分析。