U937 cells with CRISPR interference at regulatory genetic variants [ATAC-seq]

Gene enhancers often form long-range contacts with promoters, but it remains unclear if enhancer activity and their chromosomal contacts are mediated by the same DNA sequences and recruited factors. Here, we study the effects of expression quantitative trait loci (eQTLs) on enhancer activity and promoter contacts in primary monocytes isolated from 34 individuals. Using eQTL-Capture Hi-C and a Bayesian approach considering both intra- and inter-individual variation, we detect 19 eQTLs associated with enhancer-eGene promoter contacts, most of which also associated with enhancer accessibility and activity. Capitalising on these shared effects, we devise a multi-modality Bayesian strategy, which identifies 629 “trimodal QTLs” jointly associated with enhancer accessibility, eGene promoter contact, and gene expression. Causal mediation analysis and CRISPR interference reveal causal relationships between these three modalities. Many detected QTLs overlap disease susceptibility loci and influence the predicted binding of myeloid transcription factors, including SPI1, GABPB and STAT3. Additionally, a variant associated with PCK2 promoter contact directly disrupts a CTCF binding motif and impacts promoter insulation from downstream enhancers. Jointly, our findings suggest an inherent genetic coupling between enhancer activity and connectivity with relevance for human disease, and highlight the role of genetically-determined chromatin boundaries in gene control. Overall design: This study focused on the effects of regulatory genetic variants (cQTLs, as described in the publication) on enhancer function. The data in this repository comprise a CRISPRi experiment where dCas9 KRAB was directed to cQTLs, associated with expression of THBS1, in U937 monocyte-like cells. The sequencing outputs were 1) ATAC-seq, as a proxy of enhancer/promoter activity, and 2) 4C-seq, to determine the effect on 3D promity with the THBS1 promoter.