Single cell level analysis of the Arabidopsis root transcriptome and chromatin accessibility. Single cell level analysis of the Arabidopsis root transcriptome and chromatin accessibility

The nuclei of Arabidopsis root seedlings were isolated then use to establish a single cell level resolution transcriptome (sNucRNA-seq) and profile of chromatin accessibility (sNucATAC-seq) applying 10X Genomics technologies. Overall design: The nuclei of the root of 7 days-old Arabidopsis seedlings grown on agar MS medium were isolated. Upon isolation, the nuclei suspension was used to create sNucRNA-seq and sNucATAC-seq libraries. Five and two independent replicates were generated to perform these experiments., respectviely. The libraries were sequenced using high-throughput sequencing technologies. The data were analyzed using CellRanger, Seurat and Signac.