Marine Microbial Communities in Antarctic Ocean Genome sequencing. marine metagenome

The aims of this study were to i) display the microbial diversity, abundance and composition along water column of two different locations in Antarctic Ocean; ii) investigate whether the different functional groups of bacterioplankton coexist together or apart from each other in the different water depths; iii) to determine the impact of environmental factors, particularly temperature, salinity and depth on the Antarctic microbial communities. Water samples were collected at station A (47°10′ W, 61°30′ S) and station B (44°40′ W, 61°28′ S) in December 2011 and January 2012, respectively. The 16S rRNA gene hypervariable V4 region (~207 bp) was amplified using the primers: 16S-F (5’-AYTGGGYDTAAAGNG-3’) and 16S-R (5’-TACNVGGGTATCTAATCC-3’) (16). Sequencing was performed on an Illumina MiSeq instrument using a pair-end 150-bp sequence read run with Titanium reagents at Personal Biotechnology Company (Shanghai, China)