Phytophthora Ic clade sequencing

We sequenced fourteen isolates of Ic clade species. DNA was extracted from these isolates using the CTAB method from mycelial tissue. Sequencing was done in a paired-end format using an Illumina NovaSeq platform. Additionally, we sequenced four isolates from different Ic clade species (P. infestans RS2009P1; P. mirabilis P144; P. ipomoeae PIC99167; P. phaseoli F_18), using long-read sequencing and the Oxford Nanopore MinION platform with a R9.4.1 flow cell and the SQK-LSK110 Ligation Sequencing Kit. DNA extractions for the MinION sequences were performed with a QIAGEN DNeasy Plant Mini Kit following the manufacturers instructions. Sequencing was done with the Ligation Sequencing Kit (SQK-LSK110), Agencourt AMPure XP beads (Beckman Coulter, A63881), and the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (E7180S) following the manufacturers instructions.