Size fractionation for total Chl a within the surface layer and calculated size distribution of total Chl a from discrete bottle samples collected during CCE LTER process cruises in the CCE region, 2006 - 2017 (ongoing).

Water for size fractionation of chlorophyll a is sampled from ~10m depth (surface layer) in the CCE study area. The size distribution of total chlorophyll a is determined by filtering water though filters of differing pore sizes. These are then extracted in acetone and analyzed fluorometrically with Turner Designs 10-AU Fluorometer on CCE Process cruises (since 2006, ongoing). Chlorophyll a and taxon-specific pigments (chlorophylls and carotenoids) are qualitatively and quantitatively characterized in the lab onshore by several size fractions (< 1µm to > 20µm) utilizing High Performance Liquid Chromatography (HPLC) analysis. The samples analyzed within the CCE region are used to develop a metric for phytoplankton community structure that can be used to monitor its state and changes thereof over time.

用于叶绿素a粒径分级分析的水样采自CCE研究区域约10米深度的表层水体。总叶绿素a的粒径分布通过不同孔径滤膜过滤水样的方式测定，随后将滤膜置于丙酮中萃取，并在CCE过程航次中使用Turner Designs 10-AU型荧光计开展荧光光度法分析（该工作自2006年起启动，目前仍在持续进行）。在岸基实验室中，研究人员利用高效液相色谱（High Performance Liquid Chromatography, HPLC）技术，对<1μm至>20μm多个粒径分级的叶绿素a及类群特异性色素（叶绿素类与类胡萝卜素类）完成定性与定量表征。本CCE区域内的分析样本被用于构建浮游植物群落结构评估指标，该指标可用于长期监测浮游植物群落的状态及其随时间的动态变化。