Properties of deletions that inhibit de novo induction of [PSI+].

All strains contain the [PIN+] prion. Deletion strains were assessed compared to wildtype. (-) indicates a lower degree compared to wildtype. Whether strains exhibited enhanced or reduced toxicity during Sup35PD overexpression in Tyedmers et al. [46] is listed under ‘Toxicity with Sup35PD overexpression’. ‘Reaches stationary phase after 24 hours’ indicates which deletion strains containing Sup35PD-GFP, when induced with copper for 24 hours, reach stationary phase (Figure S3). Strains carrying a deletion of LAS17 took 36 hours to reach stationary phase. ‘Maintains [PIN+]’ and ‘Maintains [PSI+]’ indicate strains that can propagate the respective prion after cytoduction. ‘[PSI+] induction’ indicates the relative induced frequency of [PSI+] formation (Figure 2). ‘Detection of Sup35PD-GFP rings’ indicates the fraction of cells containing rings after 24 hours of Sup35PD-GFP overexpression (Figure 3A), whereas the ‘viability of ring cells’ refers to toxicity associated with ring containing cells (Figure 3B). The frequency of cells with bright 103Q-GFP aggregates accumulating after one to two hours of induction (Figure 4B) are summarized under “103Q bright aggregates.” The effect of 103Q-GFP overexpression on the growth of deletions strains are under ‘Growth of [PIN+] 103Q-GFP expressing cells’.aaggregates were reduced, but faint aggregates on diffuse background were increased.borted previously by [52].