Intracellular zinc chaperones in plants

Metal availability is tightly regulated within cells through metal homeostasis to prevent cytotoxicity from excess and protein inactivity from deficiency. Limited bioavailability of essential metals in soil can negatively impact crop health and yield. A plant zinc (Zn)-homeostatic mechanism involving Zn transferases has been discovered, which plays a crucial role in delivering zinc to vital zinc-dependent proteins, particularly under zinc limitation. This study focused on investigating the effects of loss of AtZNG1 (AT1G26520), a key component of this mechanism, on growth, transcriptome, and proteome in Arabidopsis thaliana during zinc deficiency. The findings from multi-omics analyses highlight the broad consequences of this mechanism's disruption. The goal of this experiment wass to identify differentially abundant proteins in A.thaliana root tissue upon Zn withdrawal, in S999 zng1 mutant cells and wild-type control.