Gene expression profiling of fetal liver-derived hematopoietic stem cells in conditional ESAM-knockout mice.

Endothelial cell-selective adhesion molecule (ESAM) is a life-long maker for hematopoietic stem cells (HSCs). Although we previously elucidated the functional importance of ESAM on HSCs in stress-induced hematopoiesis in adults, it remained unknown how ESAM affects hematopoietic development during fetal life. To address this issue, we analyzed fetuses of conditional ESAM-knockout mice. LSK CD48- cells of E14 healthy FLs, on which high amount of ESAM is uniformly expressed, were incubated with or without a rat mAb against mouse ESAM. Then, the cells were incubated with goat anti-rat IgG Abs-conjugated microbeads for 4 hrs and were subsequently applied to RNA-sequencing analyses.