Description and evaluation of experimental models for uterine transplantation in pigs

ABSTRACT Objective: To evaluate the technique of uterine transplantation and the use of drugs used in the process of immunosuppression. Methods: We included 12 sows, and immunosuppression was performed with minimal doses of cyclosporine, and cross-match was done to exclude the possibility of blood incompatibility. Hysterectomy was performed in the donor under general anesthesia, with the removal of the aorta and inferior vena cava in monobloc, and anastomosis of these vessels was made in the recipient. Results: Six experiments were performed, and on the immediate postoperative period, five animals had good reperfusion. However, on the seventh postoperative day, histological analysis showed rejection in five animals. Conclusion: The experimental model of uterine transplantation is feasible, but monitoring doses of immunosuppressants is pivotal to prevent rejection episodes.摘要 目的：评估子宫移植（uterine transplantation）技术及免疫抑制（immunosuppression）过程中所用药物的应用情况。 方法：纳入12头母猪，采用最低剂量环孢素（cyclosporine）进行免疫抑制，并实施交叉配型（cross-match）以排除血液不相容的可能性。对供体实施全身麻醉下的子宫切除术（hysterectomy），整块切取主动脉（aorta）与下腔静脉（inferior vena cava），并在受体体内完成上述血管的吻合术（anastomosis）。 结果：共完成6组实验，术后即刻有5头动物实现良好的血管再灌注（reperfusion）。但在术后第7天，组织学分析（histological analysis）显示5头动物出现排斥反应（rejection）。 结论：子宫移植实验模型具备可行性，但监测免疫抑制剂剂量对于预防排斥反应发作至关重要。