Introduction: Serologic characterization of anti-human platelet antigen (HPA) alloantibodies is crucial in fetal neonatal alloimmune thrombocytopenia (FNAIT). The gold standard MAIPA assay requires fr
ChIP-seq assay was performed with anti-p45 antibody using in vitro cultured primary megakaryocytes to identify direct p45 target genes in megakaryocytes. This analysis revealed genes that regulate pl
Whole fetal livers were collected from mouse fetuses at embryonic day 14.5 (E14.5), and single-cell suspensions were prepared by successive passage through 18-, 21 and 23-gauge needles. Fetal liver ce
Downregulation of GATA-2 in megakaryocytic cells G1ME caused cell cycle arrest. To identify GATA-2 target genes, we transduced G1ME cells with control vector or a vector expressing shRNA specific for