iPS derived microglia like cells cultured with human neurons and astrocytes

In this article, Jose Polo and colleagues molecularly compare existing microglia differentiation methods from stem cells, generate a new dual fluorescent and enzymatic reporter tool to study microglia in vitro and in vivo and show that human astrocyte/neuron co-culture pushes the regulatory landscape of in vitro microglia-like cells to more closely resemble bona fide microglia. Overall design: RNA-seq experiment examination of iPS-derived microglia like cells cultured in presence (or not) of human astrocytes/neurons, or cholesterol