Additional file 2: of Quadruplex DNA in long terminal repeats in maize LTR retrotransposons inhibits the expression of a reporter gene in yeast

Overview of oligonucleotides, BAC clones and primers used in study. Name is derived from Maize TE Database. BAC referes to ZMMBBc library coordinates of clones containing given element. G4 motif coresponds to oligonucleotides used for CD measurments and UV melting. Guanine tracks and mutations are highlighted by bold bigger font in wilde types and mutants respectively. Forward and reverse primers were used for element amplification from BAC clones, the product length is indicated as last colum. Mutagenic primers were used for generating constructs with mutatnt LTRs, mutations are highlighted as mentiond above. RACE and RACE nested primers were used for rapid amlification of cDNA ends in yeast and maize. (XLSX 10 kb)

本研究中使用的寡核苷酸（oligonucleotides）、细菌人工染色体（BAC）克隆与引物（primers）概况。其命名源自玉米转座因子数据库（Maize TE Database）。BAC指包含目标转座因子的克隆在ZMMBBc文库中的坐标信息。G4基序（G4 motif）对应用于圆二色谱（CD）测量与紫外熔融（UV melting）实验的寡核苷酸。野生型与突变体中的鸟苷序列段及突变位点分别以加粗大号字体标注。正向引物与反向引物用于从BAC克隆中扩增目标转座因子，产物长度列于表格最后一列。诱变引物用于构建携带突变长末端重复序列（mutant LTRs）的基因构建体，突变位点标注方式如前所述。用于快速扩增cDNA末端（RACE）的巢式引物（nested primers）则用于酵母与玉米中的cDNA末端快速扩增实验。(XLSX 10 kb)