Cotton Late Embryogenesis Abundant (LEA2) genes promote Root growth and Confers Drought Stress Tolerance in Transgenic Arabidopsis thaliana

<b>Supplementary Fig. S1</b>: Phylogenetic tree, gene
structure and motif compositions of <i>LEA2</i>
genes in upland cotton. The phylogenetic tree was constructed using MEGA 7.0.
Exon/intron structures of <i>LEA </i>genes
in upland cotton, exons introns and up/down-stream were represented by yellow
boxes, black lines and blue boxes, respectively. Protein motif analysis
represented by different colours, and each motif represented by number; <b>Supplementary Fig. S2</b>: conserved motifs
in upland cotton, <i>G. hirsutum</i> LEA2
proteins. The conserved motifs were obtained using MEME program. The color
scheme of the logo indicates amino acid types. Polar: green=uncharged;
blue=+vely charged; red=-vely charged; Non-polar: violet/purple=aliphatic. As
described by Dure, 2001; <b>Supplementary Fig.
S3</b>: Alignment of the LEA2 protein sequences of Upland cotton, <i>Gossypium hirsutum</i>. Amino acid sequences
were aligned using the ClustalW2 algorithm. Dashes indicate gaps introduced for
optimal alignment. The typical LEA2 sequence elements are bound in boxes in
different colours: K segment–red box; Y segment–green; S segment–black; E
segment–gray; R segment–purple; D segment–yellow; <b>Supplementary Fig. S4</b>. <i>LEA2 </i>genes
distribution in A and D cotton chromosomes: Chromosomal position of each <i>LEA</i>2 genes was mapped according to the upland cotton genome. The chromosome
number is indicated at the top of each chromosome; <b>Supplementary Fig. S5</b>: <i>LEA2</i>
genes distribution in upland cotton chromosomes. Chromosomal position of each<i> LEA</i>2 genes was mapped according to the upland cotton genome. The chromosome
number is indicated at the top of each chromosome; <b>Supplementary Table S1</b>: List of primers used for upland cotton, <i>G. hirsutum</i> <i>LEA2</i> genes expression analysis under drought stress; <b>Supplementary Table S2</b>: Conserved
domain analysis of LEA2s proteins identified from upland cotton using CDD tool
from NCBI; <b>Supplementary Table S3</b>:
Transmembrane domains in LEA2s identified from upland cotton, using TMHMM and
SOSUI servers. ExpAA: The expected number of amino acids in transmembrane
helices. First60: The expected number of amino acids in transmembrane helices
in the first 60 amino acids of the protein. MP: Membrane protein; TMH: The
number of predicted transmembrane helices; <b>Supplementary
Table S4</b>: Subcellular location as determined by TargetP and Pprowler
prediction tools. SP-secretory pathways; C-chloroplast;-any other location; <b>Supplementary Table S5</b>: Gene
duplication and determination of the Ks/Ka in paralogous gene pairs; <b>Supplementary</b> <b>Table S6</b>: Cis element analysis of putative LEA 2 promoters related
to drought stress and <b>Supplementary
Table S7</b>: <i>LEA2</i> genes and miRNA
targets