Single Cell Smart-Seq 3 RNA-Seq and Bulk Exome Seq from Breast Cancer Patients

Data Set DescriptionSingle cell RNA sequencing (Samrt-Seq3) and Whole exome sequencing from multiple regions of individual tumors from Breast Cancer patients and also single cell RNA seq for two ovarian cancer cell lines.The dataset contains raw sequencing data for various high-throughput molecular tests performed on two sample types: tumor samples from two breast cancer patients and cell lines derived from High-grade serous carcinoma Patients. The breast cancer data comes from two patients: patient 1 (BCSA1) has two tumor regions A-B and patient 2 (BCSA2) has five regions(A-E). For a normal sample and each region from each patient Whole Exome Sequencing was performed using Twist Biosciences Human Exome Kit by the SNP&SEQ Technology platform, SciLifeLab, National Genomics Infrastructure Uppsala, Sweden. Also for each patient, EPCAM+ CD45- sorted cells from all the regions where sorted to a 384 well plate, and Smart-Seq3 libraries were prepared at Karolinska Institutet and sequenced at National Genomics Infrastructure Uppsala, Sweden.The HGSOC cell-line data comes from OV2295R2 and TOV2295R cell lines described in Laks et al Cell 2019 Nov 14; 179(5): 1207–1221.e22 doi: 10.1016/j.cell.2019.10.026 . The cell line Smart-Seq3 libraries were prepared from two 384 well plates at Karolinska Institutet and sequenced at National Genomics Infrastructure Uppsala, Sweden.Terms for accessThis dataset is to be used for research on intratumor heterogeneity and subclonal evolution of tumors. To apply for conditional access to the dataset in this publication, please contact [e-mail].

数据集描述：本数据集汇集了来自乳腺癌患者多区域肿瘤的单一细胞RNA测序（Smart-Seq3）和全外显子测序数据。数据集包含了针对两种样本类型的高通量分子测试的原始测序数据：来自两名乳腺癌患者的肿瘤样本以及源自高级别浆液性癌患者的细胞系。乳腺癌数据来源于两名患者：患者1（BCSA1）包含两个肿瘤区域A-B，患者2（BCSA2）包含五个区域（A-E）。对于每个患者的正常样本及其各自的区域，均采用Twist Biosciences Human Exome Kit通过SNP&SEQ技术平台，SciLifeLab，瑞典乌普萨拉国家基因组学基础设施进行了全外显子测序。此外，针对每个患者，来自所有区域的EPCAM+ CD45- 分选细胞被分选至384孔板，并在卡罗林斯卡学院制备Smart-Seq3文库，并在瑞典乌普萨拉国家基因组学基础设施进行测序。HGSOC细胞系数据来源于Laks等人在2019年11月14日发表于《细胞》杂志的OV2295R2和TOV2295R细胞系（doi: 10.1016/j.cell.2019.10.026）。细胞系Smart-Seq3文库在卡罗林斯卡学院从两个384孔板中制备，并在瑞典乌普萨拉国家基因组学基础设施进行测序。访问条款：本数据集旨在用于研究肿瘤的肿瘤内异质性和亚克隆演化。如需申请条件性访问本数据集，请联系[e-mail]。