Cancer-specific miRNAs Extracted from Tissue-exudative Ex-tracellular Vesicles in Ovarian Clear Cell Carcinoma

Ovarian clear cell carcinomas (OCCs) arise from endometriotic cysts that many women develop. Because the prognosis for OCC is poor, there is a need to identify biomarkers for early detection of OCC. Extracellular vesicles have attracted attention as biomarker carriers. This study aimed to identify cancer-specific miRNAs as novel OCC biomarkers using tissue-exudative extracellular vesicles (Te-EVs). Te-EVs were collected from patients with OCC on one side and a normal ovary on the other side. RNA sequencing was performed to identify cancerspecific miRNAs in Te-EVs. We identified tissue-specific miRNAs in the EVs secreted by OCC tissues. These EV miRNAs can be used as biomarkers for the early diagnosis and detection of OCC. Specimens were collected from four patients with Ovarian Clear Cell Carcinomas (OCCs); all four had OCCs on one side and normal ovaries on the other side. miRNAs extracted from EVs secreted from OCCs were compared with those extracted from EVs secreted from normal tissue.