Osmotic parameters of glioblastoma cell lines.

The isotonic cell radius a was determined by video microscopy from the cross-sections of cells, such as shown in Figures 4A and 4D. The osmotically inactive volume fraction β was determined from the Boyle van’t Hoff plots (Figure S4). The osmotic water permeability Pw and the solute permeability during RVD α were determined by fitting the Lúcio-model [50] to the volumetric data obtained with N cells (RHS column) in 100-mOsm sucrose solution (Figure 5A).