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Processed mosaic of a sagittal section through the brain of mouse model of glioma. To produce transformed cells, creGFAP mice were injected in the cortex with Ras/s.i.p53 virus and perfused after 5 weeks. In this system, GFP is introduced by the virus and expression is induced by an IRES site in the mRNA. Thus, the GFP gene is expressed whenever the virus infects a cell expressing GFAP (green). The section was immunolabeled for GFAP (red) and counterstained with DAPI (blue). See MP:8063 for a section from the same brain labeled with nestin.本数据集为胶质瘤小鼠模型大脑矢状切面的经处理拼接图像。为获得转化细胞，研究人员向creGFAP小鼠（creGFAP mice）的皮层注射Ras/s.i.p53病毒，并于造模后5周对小鼠实施灌流处死。本实验体系中，病毒携载绿色荧光蛋白（GFP），其表达由mRNA内的内部核糖体进入位点（internal ribosome entry site）介导。当病毒感染表达胶质纤维酸性蛋白（glial fibrillary acidic protein）的细胞时，即可启动GFP的表达，呈现绿色荧光信号。该脑组织切片使用抗GFAP抗体进行免疫荧光标记，呈现红色信号；并以4',6-二脒基-2-苯基吲哚（DAPI）进行复染，呈现蓝色信号。如需查看同一脑组织中经巢蛋白（nestin）标记的切片，请参阅编号为MP:8063的样本。