Integrated Analysis of Comparative Lipidomics and Proteomics Reveals the Dynamic Changes of Lipid Molecular Species in High-Oleic Acid Peanut Seed

Modern peanut contains fatty acid desaturase 2 (FAD2) mutation, which is capable of producing high oleic
acid for human
health. However, the dynamic changes of the lipidome regarding fad2 remain elusive in peanut seed. In the present study,
547 lipid features were identified in high- and normal-oleic peanut
seeds by utilizing the mass spectrometric approach. The fad2-induced differently expressed lipids (DELs) were polarly distributed
at early and maturation stages during high-oleic acid (OA) seed development.
Subsequently, integration of previously published proteomic data and
lipidomic data revealed that 21 proteins and 149 DELs were annotated
into the triacylglycerol assembly map, of which nine enzymes and 31
lipid species shared similar variation tendencies. Additionally, the
variation tendencies of 17 acyl fatty acids were described in a hypothetical
biosynthetic pathway. Collectively, the understanding of the lipid
composition correlated with fad2 established a foundation
for future high-OA peanut breeding based on lipidomic data.