Transcriptional profiles of Daptomycin-resistant S.aureus (CBX67). unidentified

The aim is to know transcriptional profile change in Daptomycin-resistant S.aureus versus its vancomycin-susceptible counterpart. Two pairs comparison were performed: 1) Daptomycin-susceptible wild type N315deltaIP and its daptomycin-resistant derivative N315deltaIP-10*3d1, 2) Daptomycin-susceptible wild type N315deltaIP and Daptomyin-susceptible revertant N315deltaIP-10*3d1-10th, derived from daptomycin-resistant strain N315deltaIP-10*3d1. Daptomycin-resistant strain N315deltaIP-10*3d1 is developed by exposing daptomycin-susceptible MRSA strain N315deltaIP to daptomycin with gradual increment of daptomycin concentration. The Daptomyin-susceptible revertant N315deltaIP-10*3d1-10th was generated by drug-free passage of N315deltaIP-10*3d1 to 10 days. RNA extraction and microarray hybridization was performed as described in Ref.1.