Single cell analysis of the impact of intermediate passaging on cell fate determination of mesendoderm progenitors

To investigate the impact of intermediate passaging on cell fate determination, cells were passaged during mesendoderm differentiation, and samples collected at different time points were subjected to single cell RNA sequencing. We report here that intermediate passaging alters cell fate and directs mesendodermal progenitors toward cardiac cell fate. Single cell RNA sequencing on samples collected on Day 2, 3 and 10 of differentiation, comparing passaged and non-passaged cells in the presence and absence of WNT inhibitor.