Gene expression microarray analysis on hippocampus of Schnurri-2 knockout and wild-type control mice

Schnurri-2 (Shn-2), an NF-kappa B site-binding protein, tightly binds to the enhancers of major histocompatibility complex (MHC) class I genes and inflammatory cytokines, which have been shown to harbor common variant single nucleotide polymorphisms associated with schizophrenia. Shn-2 knockout mice show behavioral abnormalities that strongly resemble those of schizophrenics. We performed gene expression microarray analysis of hippocampi from Shn-2 knockout and wild-type control mice. Hippocampal RNA isolated from four Shn-2 knockout and four control wild-type mice were compared.Schnurri-2（Shn-2）作为核因子κB（NF-kappa B）位点结合蛋白，可紧密结合主要组织相容性复合体（MHC）I类基因与炎症细胞因子的增强子序列；现有研究证实，此类序列中存在与精神分裂症相关的常见变异型单核苷酸多态性。Shn-2基因敲除小鼠会表现出与精神分裂症患者高度相似的行为异常。本研究对Shn-2基因敲除小鼠与野生型对照小鼠的海马体开展了基因表达微阵列分析，并对4只Shn-2基因敲除小鼠与4只野生型对照小鼠分离得到的海马体核糖核酸（RNA）进行了对比分析。