Labcode 6A- HIV neutralization Protocol using Ghost cells

This protocol describes the preparation and titration of pseudovirus (PV) and the detection of neutralizing activity of monoclonal antibodies (mAb) as well as sera/plasma obtained from HIV infected individuals using Ghost cells as target cells.本实验方案详述了假病毒（pseudovirus, PV）的制备与滴定方法，以及以Ghost细胞（Ghost cells）为靶细胞，检测单克隆抗体（monoclonal antibodies, mAb）与HIV感染者血清/血浆中和活性的完整流程。