RNA-Seq experiments performed on rat Schwann (S16) cells

We utilized RNA-Seq on rat Schwann (S16) cells to determine global gene expression. This information was generated as part of a larger effort to characterize cis-regulatory elements and global gene expression within Schwann cells. To achieve this, we generated RPKM values across two independent biological replicates. This dataset was also used to predict cis-regulatory element function on genes following CRISPR knockout studies.本研究采用RNA测序（RNA-Seq）技术对大鼠雪旺氏（Schwann）细胞系S16进行检测，以分析其全基因组基因表达谱。该测序数据属于一项更大规模研究的产出成果，该研究旨在系统表征雪旺氏细胞内的顺式调控元件（cis-regulatory elements）与全基因组基因表达谱。为完成该研究目标，我们针对两组独立生物学重复样本生成了RPKM值。本数据集还被用于在CRISPR基因敲除（CRISPR knockout）实验后预测基因的顺式调控元件功能。