Supplementary material 1: Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR

Supplemental figure 1A and B: Agarose gel
after PCR.

A: Original image of agarose gel. B: Image
of agarose gel with virtually subtracted background.

Supplemental figure 2A and B: Agarose gel
after purification of PCR products.

A: Original image of agarose gel. B: Image
of agarose gel with virtually subtracted background.

Supplementary material 1: Protocol for Agarose
gel electrophoresis to assess PCR product yield – Comparison with
spectrophotometry, fluorometry and qPCR.

Supplementary table
1: PCR reaction composition

Supplementary table
2: PCR reaction parameters

Supplementary table
3: Primer sequences

Supplementary table 4: Peak area acquired
with ImageJ from agarose gel after PCR

Supplementary table 5: Peak area acquired
with ImageJ from agarose gel after PCR product purification

Supplemental material 3 containing: Supplementary
table 6: Results of spectrophotometry

Supplementary table 7: Results of
fluorometry

Supplementary table 8: Results of qPCR