five

Gene edition in haematopoietic cell lines

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NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/sra/ERP138898
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Due to the difficult transfection of blood cells, we developed an alternative strategy to generate CRISPR constructs. Our constructs were easily introduced into hard-to-transfect leukemic cells. Gene edition efficiencies obtained by Endonuclease T7-EI and restriction fragment length polymorphism were confirmed by sanger sequencing and next generation sequencing.
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2022-07-14
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