Contains supplementary materials and results, organized in Tables A-D and Figure A, and supplementary references.

Table A in S1 File. Clinical and pathological data of the cHL patients included in the survival analyses. Table B in S1 File. Antibodies used in the study. Table C in S1 File. Relationships between clinical characteristics and immunohistochemical variables. IHC protein expression was quantified using an automated scan, Chroma Vision Systems- ACIS III (DAKO, Glostrup, Denmark), analysing the whole area of representative tissue included in the TMAs. Associations were analysed using the Spearman test (continuous variables) or the Pearson chi-square test (categorical variables). P values are shown, grey boxes indicating significant associations (p<0.05). EXTRANODAL: extranodal disease. IPS: International Prognostic Score. STAGE IV, stage IV disease (according to the Ann Arbor classification). HB: haemoglobin level <10.5 g/dl. ALB: Serum albumin level <4 g/dl. LEUK, leucocytosis (leukocyte count >15,000/mm3). LYMPH, lymphocytopenia (lymphocyte count <600/mm3, or 8% of the leukocyte count, or both). AGE: age 45 years or older. SEX: male. DOD, death from disease. OS, overall survival (months). FFS, failure-free survival (months). OUTCOME, unfavourable versus favourable treatment response (treatment failure of first line therapy versus complete remission without relapse). Table D in S1 File. Multivariate Cox analyses. Figure A in S1 File. Western blot and Q-PCR analyses of CSF1R. (A) Western blot analyses of CSF1R protein using protein extracts from HEK293 cells transfected with either CSF1R or a closely related protein (FGFR1) as a negative control, normal tissues, cHL tumour samples, and cHL-derived cell lines. Antibody FER216 recognised a band of 100 kDa in tonsil extracts and two bands of 100 and 150 kDa in extracts from three cHL biopsies. The mAb to tubulin was used as the loading control. (B) Q-PCR detection of CSF1R and CSF1R ligand (CSF1L) mRNA from cHL tumour samples, and cHL-derived cell lines (the y-axis represents the normalized relative levels).
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