Raw qPCR data for Spiroplasma (dnaA) and D. hydei (rp49) quantification
收藏Figshare2022-04-07 更新2026-04-08 收录
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https://figshare.com/articles/dataset/Raw_qPCR_data_for_Spiroplasma_dnaA_and_D_hydei_rp49_quantification/12221069/1
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资源简介：
Roche LightCycler® 480 Instrument, 384-well output file, with 3 technical triplicates for each biological sample.<br>This file is an input for the script implementing Pfaffl Method to raw, quality checked Cp values (qPCR threshold cycles) from the Roche LightCycler® 480 Instrument, 384-well, with technical replicates for each sample.<br>Data includes two positive controls for QC (SpiroCTR & 25.1old).<br>Negative controls were removed manually at the melting curve analysis step (all showed no signs of amplification).<br>For details of output file format, see manufacturers instructions <br>http://icob.sinica.edu.tw/pubweb/bio-chem/Core Facilities/Data/R401-core/LightCycler480 II_Manual_V1.5.pdf<br><br><br>
提供机构：
Hurst, Greg; Chrostek, Ewa; Jones, Jordan; Fenton, Andy; Corbin, Chris
创建时间：
2022-04-07
