Enterobacterales plasmids - raw sequence reads

We present plasmid sequences of 21 multidrug-resistant isolates of Enterobacterales belonging to Escherichia coli (n =10), Klebsiella pneumoniae (n=9), Klebsiella oxytoca (n=1), and Citrobacter freundii (n=1). The isolates originated from effluent collected from hospital sewer pipes and from a wastewater treatment plant (WWTP) in a southwest Hungarian city. Isolation was carried out using eosin methylene blue agar supplemented with ceftriaxone and the isolates were identified with MALDI-TOF MS. Screening for multidrug resistance was conducted using four chemical classes namely, beta-lactams, aminoglycoside, fluoroquinolone, and sulfonamide. Plasmid DNA was isolated by alkaline lysis method using the Monarch plasmid DNA miniprep kit from freshly grown pure colonies. Molecular typing and Illumina sequencing of plasmid DNA ofmultiresistant strains were performed. After the assembly of identified plasmids, genes localized in plasmid sequences were determined and functionally annotated. These reconstructed plasmid sequences supplemented with gene functional annotations were deposited in the Mendeley data. Using these datasets different plasmid incompatibility groups were identified. These conjugating plasmids appear to play a key role in the transmission of multiple resistance genes in enteric bacteria via wastewater. The presented data may serve the research on the correlations between environmental antibiotic contamination and the development of bacterial resistance which causes serious problems in hospital treatments of patients