Responses of AML patient's mononuclear cells to 1,25D or ATRA.
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Cells from peripheral blood of AML patients were exposed for 96 h to 10 nM 1,25D or to 1 μM ATRA. Then the expression of CD14 antigen in 1,25D-treated blast cells was studied in flow cytometry. From remaining 1,25D-treated blasts and from ATRA-treated blasts mRNA was isolated and the expression of CYP24A1 and POR genes was studied in Real Time PCR.
创建时间:
2015-12-02



